20210318 meeting
From mn.fysikk.laglivlab
This is our meeting, welcome!
Notes from the meeting (Written by Jan Malamant)
Brainstorming:
We talked about designs:
- Incorporate some kind of heating element / cooler to control temperature. (perhaps by controlling temperature of the medium)
- Incorporate electrodes to be able to count cells.
- Incorporating multiple chambers for substances on a single chip (possibly incorporate a concentration gradient generator)
- Multiple organs on a single chip and have them interact somehow
Thomas gave us a nice introduction to how we will build a chip
- we first create a silicon wafer
- add a photoresistant resin
- Photolithography to etch designs into the wafer
- The resin will become polymerised as a result, and we can wash way any resin that isn't polymerised.
- Then we mold the design using PDMS.
We also got some nice design tips:
- Don't make small dead areas/ends
- Avoid sharp angles in the channel
- Beware of high aspect ratios to avoid collapse
- Use filters to avoid blockage in very thing sections of channels.
We were also introduced to some building blocks in microfluidics:
- Flow divide/ T-junction
- Flow focusing
- Droplet generation
- Particle size sorting
- Concentration gradient generation
- Cell counting
- Cell trapping
Problems that will arise when making designs:
- Whenever we flush the medium, we will also flush the cells. How do we avoid this? Possible solutions: Cell trapping membrane, attaching cells to the walls
- How to control gas (O2/CO2) concentration
- What kind of cells will we use?
For next session (Tuesday 23.03.2021 16.00):
- Install AutoCAD
- Make some designs using the program