20210318 meeting

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This is our meeting, welcome!

Notes from the meeting (Written by Jan Malamant)

Brainstorming:

We talked about designs:

- Incorporate some kind of heating element / cooler to control temperature. (perhaps by controlling temperature of the medium)

- Incorporate electrodes to be able to count cells.

- Incorporating multiple chambers for substances on a single chip (possibly incorporate a concentration gradient generator)

- Multiple organs on a single chip and have them interact somehow

Thomas gave us a nice introduction to how we will build a chip

  1. we first create a silicon wafer
  2. add a photoresistant resin
  3. Photolithography to etch designs into the wafer
  4. The resin will become polymerised as a result, and we can wash way any resin that isn't polymerised.
  5. Then we mold the design using PDMS.

We also got some nice design tips:

  1. Don't make small dead areas/ends
  2. Avoid sharp angles in the channel
  3. Beware of high aspect ratios to avoid collapse
  4. Use filters to avoid blockage in very thing sections of channels.

We were also introduced to some building blocks in microfluidics:

  1. Flow divide/ T-junction
  2. Flow focusing
  3. Droplet generation
  4. Particle size sorting
  5. Concentration gradient generation
  6. Cell counting
  7. Cell trapping

Problems that will arise when making designs:

  1. Whenever we flush the medium, we will also flush the cells. How do we avoid this? Possible solutions: Cell trapping membrane, attaching cells to the walls
  2. How to control gas (O2/CO2) concentration
  3. What kind of cells will we use?

For next session (Tuesday 23.03.2021 16.00):

- Install AutoCAD

- Make some designs using the program