20210622 3Dcell labnotes
From mn.fysikk.laglivlab
- PDMS was set on glass at an angle and air between PDMS and glass several places. This made me believe that PDMS had been unstuck and stuck again. I tried to redo this, but this was catastrophe. Lots of small pieces of PDMS stuck on glass and resticking did not make it water-tight. Complete waste and disaster!!!
- Agarose procedure was better:
- put agarose powder in cold water/media in Falcon tube (3 ml, 0.03 g agarose)
- let is sit and hydrate for a while => isolated particle suspension
- heated water in coffee water heater and filled a large beaker
- Falcon tube was immersed in hot water and periodically taken out to shake until liquid was clear and free of suspended particles ): agarose dissolved
- (For cells: transfer Falcon tube to 37C water, an dafter some time, add cells and stir)
- Pumping of agarose liquid at 8-15 mbar, at lower pressures it retreats. Use MUX for stop/go while keeping pressure right over limit to push forward.
- Remember to clean tubes and MUX with very hot water (>75C)right after use!
