20210705 microfluidics

PDMS

We cut out two of Thomas' chips, one 25 um and one 50 um. We put one in a jar with hexane. We filled the other one with collagen right after plasma treating it and then waited for one hour before aspirating it. The jar was too small for the PDMS chip after it started to swell so we need a bigger jar.

Passaging

We passaged the cells as usual. For one of the flasks we only changed the medium as they were not deviding very much. For the other flask we kept one half of the cells and used the other half for injecting into the chip we coated with collagen.

Injecting cells into chip

After coating the chip with collagen we waited for one hour, aspirated the chip (using the OB1) and then rinsed with PBS (using the syringe pump and the MUX).

Unfortunately we had issues with bubbles when injecting PBS, so we tried to aspirate again to try again. This unfortunately did not work.

In the mean time, the microscope turned very dark, possibly due to the lamp being misaligned with the objective, so the image is very dark and difficult to see anything.

We waited so long between injecting cells into the syringe and trying to insert them into the chip that they probably attached to the surface of the syringe, since when we injected them in, minimal cells came into the chip. We will try again later this week.

Rinsed the MUX with water and ethanol after use.