20230515

Activation of WT problem:

• characterise the rotation spectrum by also rotating the BV-2 KO and WT cells after they have been treated with an activating agent: agent inducing inflammatory response
• we want to do this because BV-2 WT might already be activated when we rotate them and we might be getting rotational spectra of activated microglial cells
• we want to make sure that the change in spectra is not due to the activation but due to the disease (build up of fatty acids)

Morphology:

• Morten concludes that there is more variation in size in KO cells than the WT cells, which is as expected
• we needed to do the morphology tests to make sure that the rotation results are not due to increase in size due to activation of the cells
• to confirm that everything is alright in terms of cell's size, we need to do the activation tests and see if the potential change in size is due to the activation
• need to keep taking images of the cell lines before splitting them for Morten's analysis

Biological duplicates:

• have multiple cell cultures of the individual cell lines: for ex 3 flasks of KO and 3 flasks of WT, and do rotation on them to get better statistics
• this is something that we can do during the summer holidays

Improving experimental results:

• check whether adding the extra chemicals from the protocol change the results of the rotation
• be more precise with cell counting during the experiments - we need to know how many cells we put into the chamber
• do the cells rotate faster when they clump up? we do experiments to confirm that

Writing the report:

• Ørjan helping us with the article: 12th of June?

To do:

• give Latifa and Catherine access to the cell lab
• send email to Ørjan
• ask Petter to thaw more cells (closer to July) - start culturing the cells from scratch
• ask Petter how many vials we have at HTH