Autoclaving

Equipment

• Autoclave scotch
• 1L tap water

For autoclaving liquids:

• Borosilicate bottle

Other:

• Glass beaker
• Aluminum foil

General procedure

1. Prepare items that will be autoclaved:
   • Liquids: Fill borosilicate bottle with solution. Untighten cap slightly and put autoclave tape across lid, touching bottle on both ends.
   • Others: Fill beaker and cover top with aluminum foil. Fasten with autoclave tape.
2. Fill autoclaver with 1L water and put items inside.
3. ...

Autoclaved water

Must be done 4-5 hours before use of water (for cooling down)

• Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the stock in 420
• Fill 1L borosilicate bottle with water
• Put cap on, untighten it slightly
• Put autoclave scotch the lid and the bottle. One piece of scotch should cover both
• Put in autoclave and follow procedure on autoclave

Autoclaving pasteur pipettes

• stock in 420 or in cupboard in 431
• put them in a 1L beaker, tips at the bottom, beware not to break any
• put some aluminum foil on top to close the beaker and fasten it into place with autoclave tape
• put it in the autoclave and follow the autoclave's procedures