Difference between revisions of "Diluted PBS"
(→Preparing diluted PBS) |
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* 500 mL volume metric flask | * 500 mL volume metric flask | ||
* 500 mL borosilicate glass bottle | * 500 mL borosilicate glass bottle | ||
| − | * PBS | + | * PBS [https://www.sigmaaldrich.com/NO/en/product/sigma/p5493 (PHOSPHATE BUFFERED SALINE 10× concentrate - P5493 from sigma / Merck) ] |
* Type II water | * Type II water | ||
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2<sup>nd</sup>: Transfer 450 ml type II water into the same above 500 ml borosilicate glass bottle. (work outside the LAF bench, on the lab bench) | 2<sup>nd</sup>: Transfer 450 ml type II water into the same above 500 ml borosilicate glass bottle. (work outside the LAF bench, on the lab bench) | ||
| − | 3<sup>rd</sup>: Autoclave the diluted PBS solution before using. | + | 3<sup>rd</sup>: [[Autoclaving | Autoclave]] the diluted PBS solution before using. |
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Step 3: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle and close the cap, then take out the bottle from LAF. | Step 3: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle and close the cap, then take out the bottle from LAF. | ||
| − | Step 4: | + | Step 4: Transfer 450 ml type II water with 500 mL volume metric flask into the 500 ml borosilicate glass bottle. (Work outside the LAF fume hood – just on lab bench). |
| − | Step 5: Autoclave the diluted PBS solution. | + | Step 5: [[Autoclaving | Autoclave]] the diluted PBS solution. |
Latest revision as of 11:48, 17 November 2023
Required Items:
- 25 mL Serological Pipette
- 500 mL volume metric flask
- 500 mL borosilicate glass bottle
- PBS (PHOSPHATE BUFFERED SALINE 10× concentrate - P5493 from sigma / Merck)
- Type II water
Preparing diluted PBS
Instruction:
1st: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle (work inside the bio LAF bench to avoid polluting the stock PBS solution)
2nd: Transfer 450 ml type II water into the same above 500 ml borosilicate glass bottle. (work outside the LAF bench, on the lab bench)
3rd: Autoclave the diluted PBS solution before using.
Work Steps
Step 1: Use 75% antibac ethanol alcohol to spray the pack surface of 25 ml Serological Pipette, the surface of the 500 ml borosilicate glass bottle and the bottle of stock solution.
Step 2: right after spray each item and place them inside the bio LAF hood.
Step 3: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle and close the cap, then take out the bottle from LAF.
Step 4: Transfer 450 ml type II water with 500 mL volume metric flask into the 500 ml borosilicate glass bottle. (Work outside the LAF fume hood – just on lab bench).
Step 5: Autoclave the diluted PBS solution.
