Difference between revisions of "20210318 meeting"
From mn.fysikk.laglivlab
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We talked about designs: | We talked about designs: | ||
− | - Incorporate some kind of heating element to control temperature. | + | - Incorporate some kind of heating element / cooler to control temperature. (perhaps by controlling temperature of the medium) |
- Incorporate electrodes to be able to count cells. | - Incorporate electrodes to be able to count cells. | ||
− | - Incorporating multiple chambers for substances on a single chip | + | - Incorporating multiple chambers for substances on a single chip (possibly incorporate a concentration gradient generator) |
− | - Multiple organs on a single chip and have them interact somehow | + | - Multiple organs on a single chip and have them interact somehow |
− | Thomas gave us a nice introduction to how we will build a chip | + | Thomas gave us a nice introduction to how we will build a chip |
# we first create a silicon wafer | # we first create a silicon wafer | ||
# add a photoresistant resin | # add a photoresistant resin | ||
Line 34: | Line 34: | ||
Problems that will arise when making designs: | Problems that will arise when making designs: | ||
− | # Whenever we flush the medium, we will also flush the cells. How do we avoid this? Possible solutions: Cell trapping membrane | + | # Whenever we flush the medium, we will also flush the cells. How do we avoid this? Possible solutions: Cell trapping membrane, attaching cells to the walls |
+ | # How to control gas (O2/CO2) concentration | ||
+ | # What kind of cells will we use? | ||
+ | |||
+ | For next session (Tuesday 23.03.2021 16.00): | ||
+ | |||
+ | - Install AutoCAD | ||
+ | |||
+ | - Make some designs using the program |
Latest revision as of 17:25, 18 March 2021
This is our meeting, welcome!
Notes from the meeting (Written by Jan Malamant)
Brainstorming:
We talked about designs:
- Incorporate some kind of heating element / cooler to control temperature. (perhaps by controlling temperature of the medium)
- Incorporate electrodes to be able to count cells.
- Incorporating multiple chambers for substances on a single chip (possibly incorporate a concentration gradient generator)
- Multiple organs on a single chip and have them interact somehow
Thomas gave us a nice introduction to how we will build a chip
- we first create a silicon wafer
- add a photoresistant resin
- Photolithography to etch designs into the wafer
- The resin will become polymerised as a result, and we can wash way any resin that isn't polymerised.
- Then we mold the design using PDMS.
We also got some nice design tips:
- Don't make small dead areas/ends
- Avoid sharp angles in the channel
- Beware of high aspect ratios to avoid collapse
- Use filters to avoid blockage in very thing sections of channels.
We were also introduced to some building blocks in microfluidics:
- Flow divide/ T-junction
- Flow focusing
- Droplet generation
- Particle size sorting
- Concentration gradient generation
- Cell counting
- Cell trapping
Problems that will arise when making designs:
- Whenever we flush the medium, we will also flush the cells. How do we avoid this? Possible solutions: Cell trapping membrane, attaching cells to the walls
- How to control gas (O2/CO2) concentration
- What kind of cells will we use?
For next session (Tuesday 23.03.2021 16.00):
- Install AutoCAD
- Make some designs using the program