Difference between revisions of "22.03.23"
From mn.fysikk.laglivlab
(Created page with "Present: Lasse, Nigar, Catherine") |
(Tag: Visual edit) |
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Present: Lasse, Nigar, Catherine | Present: Lasse, Nigar, Catherine | ||
+ | |||
+ | ==== Results (rotations per 10sec): ==== | ||
+ | Each row for a clip is a different cell. | ||
+ | {| class="wikitable" | ||
+ | !10kHz | ||
+ | !100kHz | ||
+ | !200kHz | ||
+ | !250kHz | ||
+ | !300kHz | ||
+ | !350kHz | ||
+ | !400kHz | ||
+ | !500kHz | ||
+ | !1MHz | ||
+ | !5Mhz | ||
+ | !10MHz | ||
+ | !15MHz | ||
+ | !20MHz | ||
+ | !30MHz | ||
+ | !40MHz | ||
+ | !50MHz | ||
+ | |- | ||
+ | |0.125 | ||
+ | |1 | ||
+ | |2.25 | ||
+ | |2.25 | ||
+ | |2.5 | ||
+ | |2.5 | ||
+ | |2.5 | ||
+ | |2.25 | ||
+ | |1.75 | ||
+ | |1 | ||
+ | |0.125 | ||
+ | | -0.125 | ||
+ | | -0.25 | ||
+ | | -0.75 | ||
+ | | -1 | ||
+ | | -1.25 | ||
+ | |- | ||
+ | |0.25 | ||
+ | |1.625 | ||
+ | |2.5 | ||
+ | |2.25 | ||
+ | |2.75 | ||
+ | |3 | ||
+ | |3 | ||
+ | |3 | ||
+ | |2 | ||
+ | |0.75 | ||
+ | |0.25 | ||
+ | | -0.25 | ||
+ | | -0.625 | ||
+ | | -0.75 | ||
+ | | -0.75 | ||
+ | | -1 | ||
+ | |- | ||
+ | |0.125 | ||
+ | |1.5 | ||
+ | |2 | ||
+ | |2.75 | ||
+ | |3.5 | ||
+ | |3 | ||
+ | |3.125 | ||
+ | |2.25 | ||
+ | |1.5 | ||
+ | |1 | ||
+ | |0 | ||
+ | |0 | ||
+ | | -0.25 | ||
+ | | -0.5 | ||
+ | | -1.25 | ||
+ | | -1.25 | ||
+ | |} | ||
+ | Observations: | ||
+ | |||
+ | - The characteristics seem to allign fairly well with the two previous WT experiments ([[10.03.23]] and [[15.03.23]]), so we will a spectra of the combined datasets as a control spectra to compare with the knockout (KO) cell lines | ||
+ | [[File:BV2 KO.png|thumb|720x720px|Combined rotation spectra for BV2 WT cells, extra data from 22.03.23 excluded. A total of 108 data points used.]] | ||
+ | - The extra data points did not greatly affect the spectra (peak and turning point not affected at all), but we see a kind of 'plateau' around 300-400 kHz (see green curve below). The true peak could in other words be somewhere in this range but obscured by noise. We will disregard these extra data for now and look further into it if our BV2 spectra is not accurate enough to distinguish it from KO spectra. | ||
+ | |||
+ | [[File:BV2 Rotation 3.png|left|thumb|720x720px|data plotted with previous experiments]] |
Latest revision as of 14:33, 23 March 2023
Present: Lasse, Nigar, Catherine
Results (rotations per 10sec):
Each row for a clip is a different cell.
10kHz | 100kHz | 200kHz | 250kHz | 300kHz | 350kHz | 400kHz | 500kHz | 1MHz | 5Mhz | 10MHz | 15MHz | 20MHz | 30MHz | 40MHz | 50MHz |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.125 | 1 | 2.25 | 2.25 | 2.5 | 2.5 | 2.5 | 2.25 | 1.75 | 1 | 0.125 | -0.125 | -0.25 | -0.75 | -1 | -1.25 |
0.25 | 1.625 | 2.5 | 2.25 | 2.75 | 3 | 3 | 3 | 2 | 0.75 | 0.25 | -0.25 | -0.625 | -0.75 | -0.75 | -1 |
0.125 | 1.5 | 2 | 2.75 | 3.5 | 3 | 3.125 | 2.25 | 1.5 | 1 | 0 | 0 | -0.25 | -0.5 | -1.25 | -1.25 |
Observations:
- The characteristics seem to allign fairly well with the two previous WT experiments (10.03.23 and 15.03.23), so we will a spectra of the combined datasets as a control spectra to compare with the knockout (KO) cell lines
- The extra data points did not greatly affect the spectra (peak and turning point not affected at all), but we see a kind of 'plateau' around 300-400 kHz (see green curve below). The true peak could in other words be somewhere in this range but obscured by noise. We will disregard these extra data for now and look further into it if our BV2 spectra is not accurate enough to distinguish it from KO spectra.