Difference between revisions of "Cell lab 431"

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(Cleaning and maintenance)
(Patterned gel protocol)
 
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== Scope ==
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== Usage ==
The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building
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The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.
  
== Training ==
 
 
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.
 
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.
  
== Carrying out work in the room ==
 
 
The room must be booked in [https://bookitlab.uio.no/ BookitLab]. UiO has a [https://www.uio.no/english/services/it/adm-services/bookitlab/help/index.html BookitLab user guide].
 
The room must be booked in [https://bookitlab.uio.no/ BookitLab]. UiO has a [https://www.uio.no/english/services/it/adm-services/bookitlab/help/index.html BookitLab user guide].
 
== List of things to check regularly in 431 ==
 
 
* '''<u>Sterile water:</u>'''    Always at least one full bottle of autoclaved water
 
* '''<u>75% alcohol spray:</u>''' Always at least one full spray flask of 75% alcohol
 
* '''<u>DMEM ++:</u>''' Always one half full bottle of DMEM supplied with extras
 
* '''<u>Yellow waste container:</u>''' full, clean then thoroughly before taking them in the lab
 
* '''<u>sticky mat:</u>''' Check that sticky mat sticks
 
* '''<u>Vacusafe:</u>''' Empty  when it is more than half full
 
* '''<u>Water in incubator:</u>''' Once a week, refill sterile water in incubator. Need: 1-2 l.
 
* '''<u>Diluted PBS:</u>''' When the diluted PBS bottle is less than half full prepare another
 
* '''<u>List:</u>''' Check the list of things to buy (on the wall in the fore-room)
 
* '''<u>CO2:</u>''' Check the CO2 level in bottles. Reorder bottle if necessary.
 
* '''<u>Pasteur pipettes:</u>''' Always at least 20 sterile Pasteur pipettes in hood, if not: autoclave new
 
* '''<u>Coats:</u>''' Every semester: replace coats
 
* '''<u>Water:</u>''' Change water in distilled water small containers
 
  
 
= List of maintenance procedures =
 
= List of maintenance procedures =
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* [[Vacusafe]]  
 
* [[Vacusafe]]  
 
* [[Incubator]]
 
* [[Incubator]]
* [[Cleaning]]
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* [[Cleaning V431 | Cleaning]]
 
* [[Waste disposal]]
 
* [[Waste disposal]]
 
* [[Standard items | List of standard items]]
 
* [[Standard items | List of standard items]]
  
= [https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Cell_culturing Cell culturing] =
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= Cell culturing =
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=== Cell lines ===
 
* [[Culturing Hela cells|Hela]]
 
* [[Culturing Hela cells|Hela]]
 
* [[Culturing MDCK cells|MDCK]]
 
* [[Culturing MDCK cells|MDCK]]
 
* [[Culturing RPE cells|RPE]]
 
* [[Culturing RPE cells|RPE]]
 
* [[BV-2]]
 
* [[BV-2]]
 +
* [[Muscle cells]]
  
== Collagen for 3D or coating surfaces ==
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=== Other ===
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.
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* [[Collagen | Collagen for 3D or coating surfaces]]
 
 
[https://ibidi.com/cell-culture-microscopy/107-collagen-type-i-rat-tail.html#/110-concentration_volume-5_mg_ml_4_x_5_ml Type 1 Collagen from Ibidi],  '''[https://ibidi.com/img/cms/products/cells_reagents/R_5020X_CollagenI/IN_5020X_CollagenI_05mg.pdf Protocols for use.]''' (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).
 
 
 
== [[File:Gels_patterns_20230829.odt|Patterned gel protocol]] ==
 
Previous version: [[File:Gels_patterns_20230222.docx|Patterned gel protocol]]
 
  
Original paper: [[File:Versaevel-Gabriele2014.pdf]]
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=== Protocols ===
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* [[Micropatterning on hydrogels]]

Latest revision as of 15:25, 17 November 2023

Usage

The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.

All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.

The room must be booked in BookitLab. UiO has a BookitLab user guide.

List of maintenance procedures

Preparation of solutions

Lab maintenance

Cell culturing

Cell lines

Other

Protocols