Difference between revisions of "Cell lab 431"
From mn.fysikk.laglivlab
(→Cleaning and maintenance) |
|||
(10 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | == | + | == Usage == |
− | The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building | + | The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building. |
− | |||
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form. | All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form. | ||
− | |||
The room must be booked in [https://bookitlab.uio.no/ BookitLab]. UiO has a [https://www.uio.no/english/services/it/adm-services/bookitlab/help/index.html BookitLab user guide]. | The room must be booked in [https://bookitlab.uio.no/ BookitLab]. UiO has a [https://www.uio.no/english/services/it/adm-services/bookitlab/help/index.html BookitLab user guide]. | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
= List of maintenance procedures = | = List of maintenance procedures = | ||
Line 28: | Line 10: | ||
* [[Dulbeccos Modified Eagle's Media, DMEM adding extras | DMEM]] | * [[Dulbeccos Modified Eagle's Media, DMEM adding extras | DMEM]] | ||
* [[Diluted PBS | PBS]] | * [[Diluted PBS | PBS]] | ||
+ | * [[Preparing BSA | BSA]] | ||
=== Lab maintenance === | === Lab maintenance === | ||
Line 34: | Line 17: | ||
* [[Vacusafe]] | * [[Vacusafe]] | ||
* [[Incubator]] | * [[Incubator]] | ||
− | * [[Cleaning]] | + | * [[Cleaning V431 | Cleaning]] |
* [[Waste disposal]] | * [[Waste disposal]] | ||
* [[Standard items | List of standard items]] | * [[Standard items | List of standard items]] | ||
− | = | + | = Cell culturing = |
+ | === Cell lines === | ||
* [[Culturing Hela cells|Hela]] | * [[Culturing Hela cells|Hela]] | ||
* [[Culturing MDCK cells|MDCK]] | * [[Culturing MDCK cells|MDCK]] | ||
* [[Culturing RPE cells|RPE]] | * [[Culturing RPE cells|RPE]] | ||
* [[BV-2]] | * [[BV-2]] | ||
+ | * [[Muscle cells]] | ||
− | == | + | === Other === |
− | + | * [[Collagen | Collagen for 3D or coating surfaces]] | |
− | |||
− | |||
− | |||
− | |||
− | |||
− | + | === Protocols === | |
+ | * [[Micropatterning on hydrogels]] |
Latest revision as of 15:27, 8 August 2024
Contents
Usage
The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.
The room must be booked in BookitLab. UiO has a BookitLab user guide.