Difference between revisions of "Cell lab 431"

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(List of things to check regularly in 431)
(Patterned gel protocol)
 
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== Scope ==
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== Usage ==
The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building
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The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.
  
== Training ==
 
 
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.
 
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.
  
== Carrying out work in the room ==
 
 
The room must be booked in [https://bookitlab.uio.no/ BookitLab]. UiO has a [https://www.uio.no/english/services/it/adm-services/bookitlab/help/index.html BookitLab user guide].
 
The room must be booked in [https://bookitlab.uio.no/ BookitLab]. UiO has a [https://www.uio.no/english/services/it/adm-services/bookitlab/help/index.html BookitLab user guide].
  
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* [[Vacusafe]]  
 
* [[Vacusafe]]  
 
* [[Incubator]]
 
* [[Incubator]]
* [[Cleaning]]
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* [[Cleaning V431 | Cleaning]]
 
* [[Waste disposal]]
 
* [[Waste disposal]]
 
* [[Standard items | List of standard items]]
 
* [[Standard items | List of standard items]]
  
= [https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Cell_culturing Cell culturing] =
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= Cell culturing =
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=== Cell lines ===
 
* [[Culturing Hela cells|Hela]]
 
* [[Culturing Hela cells|Hela]]
 
* [[Culturing MDCK cells|MDCK]]
 
* [[Culturing MDCK cells|MDCK]]
 
* [[Culturing RPE cells|RPE]]
 
* [[Culturing RPE cells|RPE]]
 
* [[BV-2]]
 
* [[BV-2]]
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* [[Muscle cells]]
  
== Collagen for 3D or coating surfaces ==
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=== Other ===
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.
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* [[Collagen | Collagen for 3D or coating surfaces]]
  
[https://ibidi.com/cell-culture-microscopy/107-collagen-type-i-rat-tail.html#/110-concentration_volume-5_mg_ml_4_x_5_ml Type 1 Collagen from Ibidi],  '''[https://ibidi.com/img/cms/products/cells_reagents/R_5020X_CollagenI/IN_5020X_CollagenI_05mg.pdf Protocols for use.]''' (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).
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=== Protocols ===
 
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* [[Micropatterning on hydrogels]]
== [[File:Gels_patterns_20230829.odt|Patterned gel protocol]] ==
 
Previous version: [[File:Gels_patterns_20230222.docx|Patterned gel protocol]]
 
 
 
Original paper: [[File:Versaevel-Gabriele2014.pdf]]
 

Latest revision as of 15:25, 17 November 2023

Usage

The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.

All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.

The room must be booked in BookitLab. UiO has a BookitLab user guide.

List of maintenance procedures

Preparation of solutions

Lab maintenance

Cell culturing

Cell lines

Other

Protocols