Difference between revisions of "22.03.23"

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Observations:
 
Observations:
  
- first impression, behaviour seems similar to previous WT experiments
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- The characteristics seem to allign fairly well with the two previous WT experiments ([[10.03.23]] and [[15.03.23]]), so we will a spectra of the combined datasets as a control spectra to compare with the knockout (KO) cell lines
 +
[[File:BV2 KO.png|thumb|720x720px|Combined rotation spectra for BV2 WT cells]]
 +
- The extra data points did not greatly affect the spectra (peak and turning point not affected at all), but we see a kind of 'plateau' around 300-400 kHz (see green curve below). The true peak could in other words be somewhere in this range but obscured by noise. We will disregard these extra data for now and look further into it if our BV2 spectra is not accurate enough to distinguish it from KO spectra.
  
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+
[[File:BV2 Rotation 3.png|left|thumb|720x720px|data plotted with previous experiments]]

Revision as of 14:11, 23 March 2023

Present: Lasse, Nigar, Catherine

Results (rotations per 10sec):

Each row for a clip is a different cell.

10kHz 100kHz 200kHz 250kHz 300kHz 350kHz 400kHz 500kHz 1MHz 5Mhz 10MHz 15MHz 20MHz 30MHz 40MHz 50MHz
0.125 1 2.25 2.25 2.5 2.5 2.5 2.25 1.75 1 0.125 -0.125 -0.25 -0.75 -1 -1.25
0.25 1.625 2.5 2.25 2.75 3 3 3 2 0.75 0.25 -0.25 -0.625 -0.75 -0.75 -1
0.125 1.5 2 2.75 3.5 3 3.125 2.25 1.5 1 0 0 -0.25 -0.5 -1.25 -1.25

Observations:

- The characteristics seem to allign fairly well with the two previous WT experiments (10.03.23 and 15.03.23), so we will a spectra of the combined datasets as a control spectra to compare with the knockout (KO) cell lines

Combined rotation spectra for BV2 WT cells

- The extra data points did not greatly affect the spectra (peak and turning point not affected at all), but we see a kind of 'plateau' around 300-400 kHz (see green curve below). The true peak could in other words be somewhere in this range but obscured by noise. We will disregard these extra data for now and look further into it if our BV2 spectra is not accurate enough to distinguish it from KO spectra.

data plotted with previous experiments