Difference between revisions of "Kick-off meeting spring semester 2022"
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= Plan for spring 2022 = | = Plan for spring 2022 = | ||
== Current state of project == | == Current state of project == | ||
− | * We have some microfluidic chip designs we can use although none are perfect | + | * We have some microfluidic chip designs we can use although none are perfect (see examples below) |
+ | * Protocol for fibronectin coating is established | ||
+ | * Filling with cells works and cells do adhere. | ||
+ | * Problems with air bubbles. | ||
+ | * Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level. | ||
+ | |||
+ | == Goal for semester == | ||
+ | Make a device that fulfills the following | ||
+ | * Controls CO2 level | ||
+ | ** CO2 input can be quickly changed | ||
+ | ** CO2 concentration is measured | ||
+ | ** feedback from measurement to CO2 input | ||
+ | * Cells adhere | ||
+ | * Cells stay alive and divide until they are confluent | ||
+ | * Splitting: partial cell release by trypsination and increased flow rate | ||
+ | * Cells continue to adhere and divide after splitting | ||
+ | Write a scientific paper | ||
+ | |||
[[File:Anniken network.png|left|thumb|Network with gradient generator]] | [[File:Anniken network.png|left|thumb|Network with gradient generator]] | ||
[[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] | [[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] |
Revision as of 15:09, 27 January 2022
Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag
Plan for spring 2022
Current state of project
- We have some microfluidic chip designs we can use although none are perfect (see examples below)
- Protocol for fibronectin coating is established
- Filling with cells works and cells do adhere.
- Problems with air bubbles.
- Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.
Goal for semester
Make a device that fulfills the following
- Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
- Cells adhere
- Cells stay alive and divide until they are confluent
- Splitting: partial cell release by trypsination and increased flow rate
- Cells continue to adhere and divide after splitting
Write a scientific paper