Difference between revisions of "Kick-off meeting spring semester 2022"
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* Splitting: partial cell release by trypsination and increased flow rate | * Splitting: partial cell release by trypsination and increased flow rate | ||
* Cells continue to adhere and divide after splitting | * Cells continue to adhere and divide after splitting | ||
+ | * Possibly perform an additional experimentlike | ||
+ | ** migration assay by entering SDF-1/CXCL12 into a side channel | ||
+ | ** Ca2+ activation by flow, PBS and other changes | ||
Write a scientific paper | Write a scientific paper | ||
[[File:Anniken network.png|left|thumb|Network with gradient generator]] | [[File:Anniken network.png|left|thumb|Network with gradient generator]] | ||
[[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] | [[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] |
Revision as of 15:21, 27 January 2022
Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag
Plan for spring 2022
Current state of project
- We have some microfluidic chip designs we can use although none are perfect (see examples below)
- Protocol for fibronectin coating is established
- Filling with cells works and cells do adhere.
- Problems with air bubbles.
- Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.
Goal for semester
Make a device that fulfills the following
- Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
- Cells adhere
- Cells stay alive and divide until they are confluent
- Splitting: partial cell release by trypsination and increased flow rate
- Cells continue to adhere and divide after splitting
- Possibly perform an additional experimentlike
- migration assay by entering SDF-1/CXCL12 into a side channel
- Ca2+ activation by flow, PBS and other changes
Write a scientific paper