Difference between revisions of "Kick-off meeting spring semester 2022"
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Dagkd@uio.no (talk | contribs) |
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ZimmerPeacock | ZimmerPeacock | ||
=== CO2 input change === | === CO2 input change === | ||
+ | Choice of method | ||
+ | * Control of CO2 in media source | ||
+ | * Control of CO2 in side channel | ||
+ | ** Choose membrane to side channel | ||
+ | *** PDMS | ||
+ | *** Gel | ||
=== Robust microfluidic protocols === | === Robust microfluidic protocols === | ||
==== Bubble-free operation ==== | ==== Bubble-free operation ==== | ||
==== Defining safe flow levels for cells ==== | ==== Defining safe flow levels for cells ==== | ||
==== Switching of different fluids ==== | ==== Switching of different fluids ==== | ||
+ | ==== Gel barrier filling ==== | ||
[[File:Anniken network.png|left|thumb|Network with gradient generator]] | [[File:Anniken network.png|left|thumb|Network with gradient generator]] | ||
[[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] | [[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] |
Revision as of 15:28, 27 January 2022
Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag
Contents
Plan for spring 2022
Current state of project
- We have some microfluidic chip designs we can use although none are perfect (see examples below)
- Protocol for fibronectin coating is established
- Filling with cells works and cells do adhere.
- Problems with air bubbles.
- Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.
Goal for semester
Make a device that fulfills the following
- Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
- Cells adhere
- Cells stay alive and divide until they are confluent
- Splitting: partial cell release by trypsination and increased flow rate
- Cells continue to adhere and divide after splitting
- Possibly perform an additional experimentlike
- migration assay by entering SDF-1/CXCL12 into a side channel
- Ca2+ activation by flow, PBS and other changes
Write a scientific paper
Specific sub-projects
CO2 measurement
ZimmerPeacock
CO2 input change
Choice of method
- Control of CO2 in media source
- Control of CO2 in side channel
- Choose membrane to side channel
- PDMS
- Gel
- Choose membrane to side channel