Difference between revisions of "10.03.23"

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Revision as of 19:08, 10 March 2023

We did our first trial rotation with BV-2 WT cells. We used 0.5ml from 10ml cell concentrate.

Observations:

  • during the recording, some cells were moving towards the electrode, which is an issue for 50 MHz, so we took 2 recordings.
  • observed rotation below 10KHz, but it was very slow so we did not record that
  • the peak was in the 100Khz range. The peak did not feel as sharp as with HeLa, more of a plateu. This will be investigated further after plotting the rotational spectra.

We are also trying to figure out the optimal cell concentration for cell culturing.

10kHz 100kHz 200kHz 300kHz 500kHz 1MHz 5Mhz 10MHz 20MHz 30MHz 40MHz 50MHz
0.25 2 2.75 3 3.25 1.5 1.25 0.625 -0.25 -1 -0.75 -1
0.125 1.5 2.75 3.5 2.5 2.25 1.25 0.25 -0.25 -0.75 -1.25 -1
0.125 1.5 3.75 3.25 2.5 2.75 0.75 0.5 -0.25 -0.5 -1 -1.25
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