Difference between revisions of "10.03.23"

Latest revision as of 13:20, 21 March 2023

We did our first trial rotation with BV-2 WT cells. We used 0.5ml from 10ml cell concentrate.

Observations:

during the recording, some cells were moving towards the electrode, which is an issue for 50 MHz, so we took 2 recordings.
observed rotation below 10KHz, but it was very slow so we did not record that
the peak was in the 100Khz range. The peak did not feel as sharp as with HeLa, more of a plateu. This will be investigated further after plotting the rotational spectra.
Spectra appears to be distinct from our HeLa spectra, but we will gather more data on this cell line before determining a control spectra

We are also trying to figure out the optimal cell concentration for cell culturing.

Each row for a clip is a different cell.

10kHz	100kHz	200kHz	300kHz	500kHz	1MHz	5Mhz	10MHz	20MHz	30MHz	40MHz	50MHz
0.25	2	2.75	3	3.25	1.5	1.25	0.625	-0.25	-1	-0.75	-1
0.125	1.5	2.75	3.5	2.5	2.25	1.25	0.25	-0.25	-0.75	-1.25	-1
0.125	1.5	3.75	3.25	2.5	2.75	0.75	0.5	-0.25	-0.5	-1	-1.25

First BV-2 WT rotation spectra (data in table above), performed by LLL electrorotation group

@@ Line 7: / Line 7: @@
 * Spectra appears to be distinct from our HeLa spectra, but we will gather more data on this cell line before determining a control spectra
 We are also trying to figure out the optimal cell concentration for cell culturing.
+==== Results (rotations per 10sec): ====
+Each row for a clip is a different cell.
 {| class="wikitable"
 !10kHz