Difference between revisions of "Autoclaving"

Latest revision as of 12:06, 17 November 2023

For autoclaving liquids:

Other:

Prepare items that will be autoclaved:
- Liquids: Fill borosilicate bottle with solution. Untighten cap slightly and put autoclave tape across lid, touching bottle on both ends.
- Others: Fill beaker and cover top with aluminum foil. Fasten with autoclave tape.
Fill autoclaver with 1L water and put items inside.
...

Must be done 4-5 hours before use of water (for cooling down)

Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the stock in 420
Fill 1L borosilicate bottle with water
Put cap on, untighten it slightly
Put autoclave scotch the lid and the bottle. One piece of scotch should cover both
Put in autoclave and follow procedure on autoclave

stock in 420 or in cupboard in 431
put them in a 1L beaker, tips at the bottom, beware not to break any
put some aluminum foil on top to close the beaker and fasten it into place with autoclave tape
put it in the autoclave and follow the autoclave's procedures

@@ Line 1: / Line 1: @@
+== Equipment ==
+* Autoclave scotch
+* 1L tap water
+For autoclaving liquids:
+* Borosilicate bottle
+Other:
+* Glass beaker
+* Aluminum foil
+== General procedure ==
+# Prepare items that will be autoclaved:
+#* Liquids: Fill borosilicate bottle with solution. Untighten cap slightly and put autoclave tape across lid, touching bottle on both ends.
+#* Others: Fill beaker and cover top with aluminum foil. Fasten with autoclave tape.
+# Fill autoclaver with 1L water and put items inside.
+# ...
 == Autoclaved water ==