Difference between revisions of "Autoclaving"
From mn.fysikk.laglivlab
(Created page with "== Autoclaved water == Must be done 4-5 hours before use of water (for cooling down) * Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the...") |
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+ | == Equipment == | ||
+ | * Autoclave scotch | ||
+ | * 1L tap water | ||
+ | |||
+ | For autoclaving liquids: | ||
+ | * Borosilicate bottle | ||
+ | Other: | ||
+ | * Glass beaker | ||
+ | * Aluminum foil | ||
+ | |||
+ | == General procedure == | ||
+ | # Prepare items that will be autoclaved: | ||
+ | #* Liquids: Fill borosilicate bottle with solution. Untighten cap slightly and put autoclave tape across lid, touching bottle on both ends. | ||
+ | #* Others: Fill beaker and cover top with aluminum foil. Fasten with autoclave tape. | ||
+ | # Fill autoclaver with 1L water and put items inside. | ||
+ | # ... | ||
+ | |||
== Autoclaved water == | == Autoclaved water == | ||
Latest revision as of 12:06, 17 November 2023
Equipment
- Autoclave scotch
- 1L tap water
For autoclaving liquids:
- Borosilicate bottle
Other:
- Glass beaker
- Aluminum foil
General procedure
- Prepare items that will be autoclaved:
- Liquids: Fill borosilicate bottle with solution. Untighten cap slightly and put autoclave tape across lid, touching bottle on both ends.
- Others: Fill beaker and cover top with aluminum foil. Fasten with autoclave tape.
- Fill autoclaver with 1L water and put items inside.
- ...
Autoclaved water
Must be done 4-5 hours before use of water (for cooling down)
- Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the stock in 420
- Fill 1L borosilicate bottle with water
- Put cap on, untighten it slightly
- Put autoclave scotch the lid and the bottle. One piece of scotch should cover both
- Put in autoclave and follow procedure on autoclave
Autoclaving pasteur pipettes
- stock in 420 or in cupboard in 431
- put them in a 1L beaker, tips at the bottom, beware not to break any
- put some aluminum foil on top to close the beaker and fasten it into place with autoclave tape
- put it in the autoclave and follow the autoclave's procedures