Difference between revisions of "Cell lab 431"
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= Cell culturing = | = Cell culturing = | ||
| + | == Cell lines == | ||
* [[Culturing Hela cells|Hela]] | * [[Culturing Hela cells|Hela]] | ||
* [[Culturing MDCK cells|MDCK]] | * [[Culturing MDCK cells|MDCK]] | ||
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* [[Muscle cells]] | * [[Muscle cells]] | ||
| + | == Other == | ||
== Collagen for 3D or coating surfaces == | == Collagen for 3D or coating surfaces == | ||
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells. | Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells. | ||
| Line 32: | Line 34: | ||
[https://ibidi.com/cell-culture-microscopy/107-collagen-type-i-rat-tail.html#/110-concentration_volume-5_mg_ml_4_x_5_ml Type 1 Collagen from Ibidi], '''[https://ibidi.com/img/cms/products/cells_reagents/R_5020X_CollagenI/IN_5020X_CollagenI_05mg.pdf Protocols for use.]''' (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021). | [https://ibidi.com/cell-culture-microscopy/107-collagen-type-i-rat-tail.html#/110-concentration_volume-5_mg_ml_4_x_5_ml Type 1 Collagen from Ibidi], '''[https://ibidi.com/img/cms/products/cells_reagents/R_5020X_CollagenI/IN_5020X_CollagenI_05mg.pdf Protocols for use.]''' (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021). | ||
| + | == Protocols == | ||
== [[File:Gels_patterns_20230829.odt|Patterned gel protocol]] == | == [[File:Gels_patterns_20230829.odt|Patterned gel protocol]] == | ||
Previous version: [[File:Gels_patterns_20230222.docx|Patterned gel protocol]] | Previous version: [[File:Gels_patterns_20230222.docx|Patterned gel protocol]] | ||
Original paper: [[File:Versaevel-Gabriele2014.pdf]] | Original paper: [[File:Versaevel-Gabriele2014.pdf]] | ||
Revision as of 15:11, 17 November 2023
Contents
Usage
The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.
All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.
The room must be booked in BookitLab. UiO has a BookitLab user guide.
List of maintenance procedures
Preparation of solutions
Lab maintenance
Cell culturing
Cell lines
Other
Collagen for 3D or coating surfaces
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.
Type 1 Collagen from Ibidi, Protocols for use. (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).
Protocols
File:Gels patterns 20230829.odt
Previous version: File:Gels patterns 20230222.docx
Original paper: File:Versaevel-Gabriele2014.pdf
