Difference between revisions of "20210513 passaging"
From mn.fysikk.laglivlab
(Created page with "Today we passaged cells. P-24. - We used a new bottle of Trypsin, which worked very well and detached all the cells within only a few minutes. Last time we passaged, we spent...") (Tag: Visual edit) |
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Today we passaged cells. P-24. | Today we passaged cells. P-24. | ||
| − | - We used a new bottle of Trypsin, which worked very well and detached all the cells within only a few minutes. Last time we passaged, we spent 30 minutes waiting for the trypsin to work. | + | - We used a new bottle of Trypsin, which worked very well and detached all the cells within only a few minutes. Last time we passaged, we spent 30 minutes waiting for the trypsin to work.We removed 9.5mL of media from the flask, so that we had 0.5mL of cells inside, and then filled each flask with 12mL of growth medium. |
Latest revision as of 13:54, 13 May 2021
Today we passaged cells. P-24.
- We used a new bottle of Trypsin, which worked very well and detached all the cells within only a few minutes. Last time we passaged, we spent 30 minutes waiting for the trypsin to work.We removed 9.5mL of media from the flask, so that we had 0.5mL of cells inside, and then filled each flask with 12mL of growth medium.
