Difference between revisions of "Kick-off meeting spring semester 2022"
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** Ca2+ activation by flow, PBS and other changes | ** Ca2+ activation by flow, PBS and other changes | ||
Write a scientific paper | Write a scientific paper | ||
+ | |||
+ | == Specific sub-projects == | ||
+ | === CO2 measurement === | ||
+ | ZimmerPeacock | ||
+ | === CO2 input change === | ||
+ | === Robust microfluidic protocols === | ||
+ | ==== Bubble-free operation ==== | ||
+ | ==== Defining safe flow levels for cells ==== | ||
+ | ==== Switching of different fluids ==== | ||
[[File:Anniken network.png|left|thumb|Network with gradient generator]] | [[File:Anniken network.png|left|thumb|Network with gradient generator]] | ||
[[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] | [[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]] |
Revision as of 15:25, 27 January 2022
Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag
Contents
Plan for spring 2022
Current state of project
- We have some microfluidic chip designs we can use although none are perfect (see examples below)
- Protocol for fibronectin coating is established
- Filling with cells works and cells do adhere.
- Problems with air bubbles.
- Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.
Goal for semester
Make a device that fulfills the following
- Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
- Cells adhere
- Cells stay alive and divide until they are confluent
- Splitting: partial cell release by trypsination and increased flow rate
- Cells continue to adhere and divide after splitting
- Possibly perform an additional experimentlike
- migration assay by entering SDF-1/CXCL12 into a side channel
- Ca2+ activation by flow, PBS and other changes
Write a scientific paper
Specific sub-projects
CO2 measurement
ZimmerPeacock