Revision as of 15:28, 27 January 2022

Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag

Plan for spring 2022

We have some microfluidic chip designs we can use although none are perfect (see examples below)
Protocol for fibronectin coating is established
Filling with cells works and cells do adhere.
Problems with air bubbles.
Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.

Make a device that fulfills the following

Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
Cells adhere
Cells stay alive and divide until they are confluent
Splitting: partial cell release by trypsination and increased flow rate
Cells continue to adhere and divide after splitting
Possibly perform an additional experimentlike
- migration assay by entering SDF-1/CXCL12 into a side channel
- Ca2+ activation by flow, PBS and other changes

Write a scientific paper

ZimmerPeacock

Choice of method

Control of CO2 in media source
Control of CO2 in side channel
- Choose membrane to side channel
  - PDMS
  - Gel

Network with gradient generator

Network with gas flow channels separated from cell area with gel channels.

@@ Line 27: / Line 27: @@
 ZimmerPeacock
 === CO2 input change ===
+Choice of method
+* Control of CO2 in media source
+* Control of CO2 in side channel
+** Choose membrane to side channel
+*** PDMS
+*** Gel
 === Robust microfluidic protocols ===
 ==== Bubble-free operation ====
 ==== Defining safe flow levels for cells ====
 ==== Switching of different fluids ====
+==== Gel barrier filling ====
 [[File:Anniken network.png|left|thumb|Network with gradient generator]]
 [[File:Gel gas flow network.png|left|thumb|Network with gas flow channels separated from cell area with gel channels.]]