Difference between revisions of "Kick-off meeting spring semester 2022"
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== Specific sub-projects == | == Specific sub-projects == | ||
=== CO2 measurement === | === CO2 measurement === | ||
− | In order to integrate a reliable CO2-measurement without too much development we will use the Zimmer & Peacock [https://www.zimmerpeacocktech.com/2021/07/16/ise-oem-electronics/ ISE OEM, pH sensor and software]. | + | In order to integrate a reliable CO2-measurement without too much development we will use the Zimmer & Peacock [https://www.zimmerpeacocktech.com/2021/07/16/ise-oem-electronics/ ISE OEM, pH sensor and software]. The first challenge will be to integrate the sensor into the medium flow, either on the chip or connecting with a tube. |
=== CO2 input change === | === CO2 input change === | ||
Choice of method | Choice of method | ||
− | * Control of CO2 in media source | + | Some background discussion is found [https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Controlling_O2_and_CO2_level here]. |
− | * Control of CO2 in side channel | + | * Control of CO2 in media source. This can only give slow changes of CO2 concentration |
+ | * Control of CO2 in side channel. This can yield quick CO2 changes. The side flow channel can be either | ||
+ | ** wet gas from gas blender | ||
+ | ** CO2-saturated media or CO2-concentration regulated media (no need for expensive gas blender, but extra flow control needed) | ||
** Choose membrane to side channel | ** Choose membrane to side channel | ||
− | *** PDMS | + | *** PDMS: simpler to avoid gel filling step, but permeability is probably lower. |
− | *** Gel | + | *** Gel: High permeability, more complex fabrication. |
+ | Papers on microfluidic systems with gas control for cell culture: | ||
+ | * [https://iopscience.iop.org/article/10.1088/1361-6439/aaa993 Wu (2018), review paper] | ||
+ | * [https://www.mdpi.com/1424-8220/17/7/1603 Bunge (2017)] | ||
+ | * [https://pubs.rsc.org/en/content/articlelanding/2013/lc/c3lc50643f Giulitti (2013)] | ||
+ | * [https://pubs.rsc.org/en/content/articlelanding/2011/lc/c1lc20505f Forry (2011)] | ||
=== Robust microfluidic protocols === | === Robust microfluidic protocols === | ||
+ | These protocols should be tested with and without cells. | ||
==== Bubble-free operation ==== | ==== Bubble-free operation ==== | ||
==== Defining safe flow levels for cells ==== | ==== Defining safe flow levels for cells ==== |
Revision as of 17:13, 27 January 2022
Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag
Contents
Plan for spring 2022
Current state of project
- We have some microfluidic chip designs we can use although none are perfect (see examples below)
- Protocol for fibronectin coating is established
- Filling with cells works and cells do adhere.
- Problems with air bubbles.
- Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.
Goal for semester
Make a device that fulfills the following
- Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
- Cells adhere
- Cells stay alive and divide until they are confluent
- Splitting: partial cell release by trypsination and increased flow rate
- Cells continue to adhere and divide after splitting
- Possibly perform an additional experimentlike
- migration assay by entering SDF-1/CXCL12 into a side channel
- Ca2+ activation by flow, PBS and other changes
Write a scientific paper. Possible journals:
- Micromachines (MDPI)
- Sensors (MDPI)
- Frontiers in Bioengineering and Biotechnology
- Biomicrofluidics
- Microfluidics and Nanofluidics
Specific sub-projects
CO2 measurement
In order to integrate a reliable CO2-measurement without too much development we will use the Zimmer & Peacock ISE OEM, pH sensor and software. The first challenge will be to integrate the sensor into the medium flow, either on the chip or connecting with a tube.
CO2 input change
Choice of method Some background discussion is found here.
- Control of CO2 in media source. This can only give slow changes of CO2 concentration
- Control of CO2 in side channel. This can yield quick CO2 changes. The side flow channel can be either
- wet gas from gas blender
- CO2-saturated media or CO2-concentration regulated media (no need for expensive gas blender, but extra flow control needed)
- Choose membrane to side channel
- PDMS: simpler to avoid gel filling step, but permeability is probably lower.
- Gel: High permeability, more complex fabrication.
Papers on microfluidic systems with gas control for cell culture:
Robust microfluidic protocols
These protocols should be tested with and without cells.