Difference between revisions of "Microfluidics"
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** Command FILLET, downarrow to get options RADIUS and POLYLINE | ** Command FILLET, downarrow to get options RADIUS and POLYLINE | ||
[[File:Skjermbilde 2021-04-20 kl. 15.33.04.png|left|thumb|Some microfluidic circuit designs for a 3" wafer]] | [[File:Skjermbilde 2021-04-20 kl. 15.33.04.png|left|thumb|Some microfluidic circuit designs for a 3" wafer]] | ||
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+ | = Photolithography = | ||
+ | [ https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Photolithography Procedures] | ||
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+ | = PDMS casting = | ||
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+ | = Assembling the chip == | ||
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+ | = Filling the chip = | ||
+ | == Water filling == | ||
+ | == Fibronectin filling == | ||
+ | == Cell filling == | ||
+ | |||
+ | = Running experiment = |
Revision as of 11:48, 3 April 2022
Contents
Designing Microfluidic masks
- Stanford microfluidics foundry has a good guide for designing your own device. That includes a guide to use AutoCAD.
- AutoCAD (a program from Autodesk) is available free.
- You can use Klayout but we have more support for using Autocad.
- Mask template for placing your designs
- This guide for designing masks has some important tips
- All fluid pathways have to be inside one or more closed contour(s)
- The outer contour should be drawn in one layer (Give it a name like "Flow")
- Any obstacles inside this outer contour must be drawn in another layer. Give it a name like "Flow inner polygons")
- Displaying your design as PDF or otherwise is not straight forward because the resolution needed. You get a fair impression using CloudConvert which is much better than AutoCads own pdf export.
- Example of a design where white lines (in screen shot below, black lines in PDF) are contours containing flow and green lines are inner polygons. Here is a PDF version of the file and the DWG file.
- Rounding corners on a region
- Command EXPLODE makes region into lines
- Command JOIN joins lines into polylines
- Command FILLET, downarrow to get options RADIUS and POLYLINE
Photolithography
[ https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Photolithography Procedures]