Difference between revisions of "Microfluidics"
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Dagkd@uio.no (talk | contribs) (→Assembling the chip =) |
Dagkd@uio.no (talk | contribs) (→Filling the chip) |
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= Filling the chip = | = Filling the chip = | ||
+ | For filling the chip with water and fibronectin use a 1ml syringe with 20G needle and 2cm long 1/16" teflon tube on the needle. | ||
== Water filling == | == Water filling == | ||
+ | * Retract <1ml distilled water into tube/needle/syringe | ||
+ | * fit in inlet of chip and fill slowly | ||
+ | * Empty syringe and needle to be ready to use for fibronectin | ||
== Fibronectin filling == | == Fibronectin filling == | ||
+ | * Take one Eppendorf with 0.5 ml fibronectin form freezer | ||
+ | * Thaw the fibronectin | ||
+ | * Retract fibronectin into tube/needle/syringe | ||
+ | * fit in inlet of chip and fill slowly | ||
+ | * | ||
== Cell filling == | == Cell filling == | ||
= Running experiment = | = Running experiment = |
Revision as of 12:53, 3 April 2022
Contents
Designing Microfluidic masks
- Stanford microfluidics foundry has a good guide for designing your own device. That includes a guide to use AutoCAD.
- AutoCAD (a program from Autodesk) is available free.
- You can use Klayout but we have more support for using Autocad.
- Mask template for placing your designs
- This guide for designing masks has some important tips
- All fluid pathways have to be inside one or more closed contour(s)
- The outer contour should be drawn in one layer (Give it a name like "Flow")
- Any obstacles inside this outer contour must be drawn in another layer. Give it a name like "Flow inner polygons")
- Displaying your design as PDF or otherwise is not straight forward because the resolution needed. You get a fair impression using CloudConvert which is much better than AutoCads own pdf export.
- Example of a design where white lines (in screen shot below, black lines in PDF) are contours containing flow and green lines are inner polygons. Here is a PDF version of the file and the DWG file.
- Rounding corners on a region
- Command EXPLODE makes region into lines
- Command JOIN joins lines into polylines
- Command FILLET, downarrow to get options RADIUS and POLYLINE
Photolithography
PDMS casting
- PDMS preparation
- Bring cup with PDMS close to wafer in Petri dish
- Pour slowly and avoid introducing air bubbles
- Remove air bubbles by puncturing them
- Fill petri dish to 5-10 mm above wafer surface
Assembling the chip
- Clean glass slide with IP and N2
- Punch holes for fluid inlets
- For 1/16" tubes use OD?? punch
- For thin teflon tube use OD?? punch
- Place both PDMS and glass slide with clean sides up on glass plate in plasma cleaner
- Follow instructions for Plasma treatment
- Place the PDMS on top of the glass slide and watch it attach
- If necessary, apply a gentle pressure to the PDMS
Filling the chip
For filling the chip with water and fibronectin use a 1ml syringe with 20G needle and 2cm long 1/16" teflon tube on the needle.
Water filling
- Retract <1ml distilled water into tube/needle/syringe
- fit in inlet of chip and fill slowly
- Empty syringe and needle to be ready to use for fibronectin
Fibronectin filling
- Take one Eppendorf with 0.5 ml fibronectin form freezer
- Thaw the fibronectin
- Retract fibronectin into tube/needle/syringe
- fit in inlet of chip and fill slowly