20210625 Microfluidics

Cell passaging

Passaged one of the flaks, the other was passaged yesterday when we needed cells. If we need any more we will use this flask.

PDMS

Cut out Thomas' and Annikens chips from the new wafer we made 21.06.2121. Plasma cleaned them and mounted them to microscope slides. We put one drop of distilled water into Thomas' chip. We put two drops (one in each inlet) of diluted collagen into Annikens chip. This worked well for covering the chip with collagen without creating Bubbles, making sure all the surface was properly covered.

Filling chip with distilled water

We filled Thomas' chip with water by using the syringe pumpe in reverse. We placed an empty 5 mL syringe into the syringe pump. Then we used one of the valves to fill one ingoing and one outgoing tube with water, making sure we had no bubbles in the tubes.