20210705 microfluidics
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Revision as of 13:54, 5 July 2021 by Annibjor@uio.no (talk | contribs)
PDMS
We cut out two of Thomas' chips, one 25 um and one 50 um. We put one in a jar with hexane. We filled the other one with collagen right after plasma treating it and then waited for one hour before aspirating it. The jar was too small for the PDMS chip after it started to swell so we need a bigger jar.
Passaging
We passaged the cells as usual. For one of the flasks we only changed the medium as they were not deviding very much. For the other flask we kept one half of the cells and used the other half for injecting into the chip we coated with collagen.
Injecting cells into chip
After coating the chip with collagen we waited for one hour, aspirated the chip (using the OB1) and then rinsed with PBS (using the syringe pump and the MUX).
