Kick-off meeting spring semester 2022
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Revision as of 17:13, 27 January 2022 by Dagkd@uio.no (talk | contribs)
Present: Kristina, Erik, Claudia, Tiril, Thomas and Dag
Contents
Plan for spring 2022
Current state of project
- We have some microfluidic chip designs we can use although none are perfect (see examples below)
- Protocol for fibronectin coating is established
- Filling with cells works and cells do adhere.
- Problems with air bubbles.
- Finite lifetime of cells. Probably because they lack oxygen and correct CO2 level.
Goal for semester
Make a device that fulfills the following
- Controls CO2 level
- CO2 input can be quickly changed
- CO2 concentration is measured
- feedback from measurement to CO2 input
- Cells adhere
- Cells stay alive and divide until they are confluent
- Splitting: partial cell release by trypsination and increased flow rate
- Cells continue to adhere and divide after splitting
- Possibly perform an additional experimentlike
- migration assay by entering SDF-1/CXCL12 into a side channel
- Ca2+ activation by flow, PBS and other changes
Write a scientific paper. Possible journals:
- Micromachines (MDPI)
- Sensors (MDPI)
- Frontiers in Bioengineering and Biotechnology
- Biomicrofluidics
- Microfluidics and Nanofluidics
Specific sub-projects
CO2 measurement
In order to integrate a reliable CO2-measurement without too much development we will use the Zimmer & Peacock ISE OEM, pH sensor and software. The first challenge will be to integrate the sensor into the medium flow, either on the chip or connecting with a tube.
CO2 input change
Choice of method Some background discussion is found here.
- Control of CO2 in media source. This can only give slow changes of CO2 concentration
- Control of CO2 in side channel. This can yield quick CO2 changes. The side flow channel can be either
- wet gas from gas blender
- CO2-saturated media or CO2-concentration regulated media (no need for expensive gas blender, but extra flow control needed)
- Choose membrane to side channel
- PDMS: simpler to avoid gel filling step, but permeability is probably lower.
- Gel: High permeability, more complex fabrication.
Papers on microfluidic systems with gas control for cell culture:
Robust microfluidic protocols
These protocols should be tested with and without cells.