User contributions
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- 12:06, 17 November 2023 (diff | hist) . . (+488) . . Autoclaving (current)
- 11:48, 17 November 2023 (diff | hist) . . (+19) . . Diluted PBS (→Preparing diluted PBS) (current)
- 11:46, 17 November 2023 (diff | hist) . . (+1) . . Diluted PBS
- 11:46, 17 November 2023 (diff | hist) . . (+75) . . Diluted PBS
- 11:43, 17 November 2023 (diff | hist) . . (-20) . . Diluted PBS
- 11:42, 17 November 2023 (diff | hist) . . (-1) . . Diluted PBS
- 11:41, 17 November 2023 (diff | hist) . . (-5) . . Diluted PBS (→Required Items:)
- 11:41, 17 November 2023 (diff | hist) . . (+5) . . Diluted PBS (→Preparing diluted PBS)
- 16:54, 16 November 2023 (diff | hist) . . (-45) . . Cell lab 431 (→Maintenance procedures)
- 16:54, 16 November 2023 (diff | hist) . . (+465) . . N Sterile water in incubator (Created page with "== Refilling water in incubator == * Put on gloves and spray gloves * Have 1-2L sterile water (autoclaved type II, not type I) ready and spray the outside * Open incubator * W...")
- 16:54, 16 November 2023 (diff | hist) . . (0) . . Cell lab 431 (→List of procedures)
- 16:53, 16 November 2023 (diff | hist) . . (+655) . . N Waste disposal (Created page with " == Waste disposal == [https://wiki.uio.no/mn/fysikk/laglivlab/images/8/86/Avfallsbehandling.pdf PDF Norwegian] place to store the Yellow waste bins. UK Floor close to the w...") (current)
- 16:53, 16 November 2023 (diff | hist) . . (+1,312) . . N Diluted PBS (Created page with "== Preparing diluted PBS == '''Instruction:''' 1<sup>st</sup>: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle (work inside the bio LAF ben...")
- 16:53, 16 November 2023 (diff | hist) . . (+1,571) . . N Dulbeccos Modified Eagle's Media, DMEM adding extras (Created page with "== Dulbeccos Modified Eagle's Media, DMEM adding extras == * It is best to prepare a few tubes with the required amount of FETAL BOVINE SERUM (FBS) and Penicilin Streptomycin...")
- 16:52, 16 November 2023 (diff | hist) . . (+707) . . N Autoclaving (Created page with "== Autoclaved water == Must be done 4-5 hours before use of water (for cooling down) * Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the...")
- 16:52, 16 November 2023 (diff | hist) . . (-402) . . Autoclaved water (→Autoclaved water)
- 16:51, 16 November 2023 (diff | hist) . . (-21) . . Cell lab 431 (→List of procedures)
- 16:51, 16 November 2023 (diff | hist) . . (+402) . . N Autoclaved water (Created page with "== Autoclaved water == Must be done 4-5 hours before use of water (for cooling down) * Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the...")
- 16:50, 16 November 2023 (diff | hist) . . (+4) . . Cell lab 431 (→List of procedures)
- 16:50, 16 November 2023 (diff | hist) . . (0) . . Vacusafe (→Items)
- 16:49, 16 November 2023 (diff | hist) . . (+6) . . Vacusafe
- 16:49, 16 November 2023 (diff | hist) . . (0) . . Vacusafe
- 16:49, 16 November 2023 (diff | hist) . . (+80) . . Vacusafe
- 16:48, 16 November 2023 (diff | hist) . . (+218) . . N Vacusafe (Created page with "== Vacusafe == * Step 1: Add 50 ml chlorine (around this amount) to the bottle. * Step 2: find one autoclave bag in v420, pour all into the bag and tight the bag. * Step3...")
- 16:47, 16 November 2023 (diff | hist) . . (+13) . . Cell lab 431
- 16:47, 16 November 2023 (diff | hist) . . (-220) . . Cell lab 431 (→Vacusafe)
- 16:47, 16 November 2023 (diff | hist) . . (+190) . . Cell lab 431 (→Maintenance procedures)
- 16:44, 16 November 2023 (diff | hist) . . (+6) . . Cell lab 431
- 16:38, 12 October 2023 (diff | hist) . . (+16) . . Cell lab 431 (Tag: Visual edit: Switched)
- 16:08, 29 August 2023 (diff | hist) . . (+78) . . Cell lab 431 (→Patterned gel protocol)
- 16:07, 29 August 2023 (diff | hist) . . (0) . . N File:Gels patterns 20230829.odt (current)
- 18:31, 3 May 2023 (diff | hist) . . (-1) . . Cell lab 431 (→Patterned gel protocol)
- 18:30, 3 May 2023 (diff | hist) . . (-145) . . Cell lab 431 (→Patterned gel protocol)
- 18:29, 3 May 2023 (diff | hist) . . (0) . . N File:Versaevel-Gabriele2014.pdf (current)
- 11:37, 3 May 2023 (diff | hist) . . (+1) . . Cell lab 431 (→Patterned gel protocol)
- 11:35, 3 May 2023 (diff | hist) . . (+3) . . Cell lab 431 (→Patterned gel protocol)
- 11:35, 3 May 2023 (diff | hist) . . (-2) . . Cell lab 431 (→Patterned gel protocol)
- 11:13, 3 May 2023 (diff | hist) . . (+196) . . Cell lab 431 (Tag: Visual edit: Switched)
- 14:33, 19 April 2023 (diff | hist) . . (+6) . . Cell lab 431 (→Patterned gel protocol)
- 14:32, 19 April 2023 (diff | hist) . . (-1) . . Cell lab 431 (→Patterned gel protocol)
- 18:26, 31 January 2023 (diff | hist) . . (+1,074) . . N Culturing MDCK cells (Created page with "MDCK is usually cultured in T25 flasks, i.e. the amounts given here are for T25 flasks (25 cm²). = Equipment = * PBS * Trypsin * DMEM + 10% FBS + 1% P/S = Protocol = # Heat...") (current)
- 18:14, 31 January 2023 (diff | hist) . . (-2) . . Cell lab 431 (→Cell culturing)
- 18:09, 31 January 2023 (diff | hist) . . (+1) . . Maintenance (→Dulbeccos Modified Eagle's Media, DMEM adding extras)
- 18:08, 31 January 2023 (diff | hist) . . (+34) . . Maintenance (→Dulbeccos Modified Eagle's Media, DMEM adding extras)
- 18:01, 31 January 2023 (diff | hist) . . (+1,141) . . N Culturing Hela cells (Created page with "=== Passaging === * Remove and discard culture medium using the vacusafe and pasteur pipette. Rinse the cells with PBS (around 10mL) and remove it. This is because the Tryps...") (current)
- 18:01, 31 January 2023 (diff | hist) . . (-126) . . Cell lab 431 (→Cell culturing)
- 17:59, 31 January 2023 (diff | hist) . . (+222) . . Cell lab 431 (→Cell culturing)
- 17:54, 31 January 2023 (diff | hist) . . (+4) . . Cell lab 431 (→List of things to check regularly in 431)
- 17:53, 31 January 2023 (diff | hist) . . (-1) . . Cell lab 431 (→Carrying out work in the room)
- 17:52, 31 January 2023 (diff | hist) . . (+27) . . Cell lab 431 (→Carrying out work in the room) (Tag: Visual edit)
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