Difference between revisions of "Main Page"
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− | + | == CEES metagenomics wiki == | |
This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature. | This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature. | ||
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== Protocols == | == Protocols == | ||
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+ | DNA extraction and 16S rDNA amplification are performed according to the protocols of the [http://www.earthmicrobiome.org/ Earth MIcrobiome Project] (EMP). The protocols can be found [http://www.earthmicrobiome.org/emp-standard-protocols/ here]. | ||
=== DNA extraction === | === DNA extraction === | ||
− | + | MoBio Powersoil HTP 96-well Soil DNA isolation kit, slightly modified by EMP: [http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/dna-extraction-protocol/ http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/dna-extraction-protocol/] | |
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+ | === 16S rDNA PCR amplification === | ||
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+ | EMP protocol: [http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/16s/ http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/16s/] | ||
− | + | == Helpful tips == | |
− | + | == Relevant articles == |
Latest revision as of 11:53, 5 March 2015
Contents
CEES metagenomics wiki
This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature.
Tips for adding a page (from the bioinf-wiki of Lex).
Material
The starting material currently includes cod gut samples, human stool samples and (...?).
Protocols
DNA extraction and 16S rDNA amplification are performed according to the protocols of the Earth MIcrobiome Project (EMP). The protocols can be found here.
DNA extraction
MoBio Powersoil HTP 96-well Soil DNA isolation kit, slightly modified by EMP: http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/dna-extraction-protocol/
16S rDNA PCR amplification
EMP protocol: http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/16s/